Signalling
Aeq

Part:BBa_K548000:Design

Designed by: Peter Qiao   Group: iGEM11_Penn   (2011-09-27)


Humanized Aequorin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 62
    Illegal NgoMIV site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence had to be modified for increased expression in human cell lines.


Source

Synthesized from GeneArt, original sequence from NCBI, optimized using GeneArt codon optimization software.

References